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. 2017 Nov 28;22:24. doi: 10.1186/s11658-017-0056-y

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© The Author(s) 2017

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 4 — Expressions of Hspb1 and HSP27 in A10 cells. a – Lentiviral infection is effective for overexpressing HSP27 in A10 cells, as shown by transfection with the vector containing the green fluorescent protein (GFP) gene, which makes the cells glow green under ultraviolet light. b – Quantitative PCR showing the Hspb1 expression level of non-infected A10 cells (non-infected control), A10 cells infected with Lenti6.3-EGFP empty vector (LVUT^EGFP), and A10 cells infected with LVUT^Hspb1 (LVUT^Hspb1). p < 0.05 between each pair of groups. Non-infected control, n = 4; LVUTE^GFP, n = 4; LVUT^Hspb1, n = 4. c – A10 cells infected with LVUT^Hspb1 express higher HSP27 protein levels than LVUT^EGFP and non-infected A10 cells. Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) was used as loading control protein