Figure 1.

Pedigree of the family under study. (A) Clinical status is indicated by open symbols (unaffected or unknown) and filled symbols (affected). Filled quadrants indicate a diagnosis as indicated in the inset legend. Histological diagnosis of carcinoma is indicated (#). A diagonal slash mark through a symbol indicates deceased. Proband is indicated by the arrow. The presence (+) or absence (−) of a mutation in tested family members is shown. (B) Detection of a mutation in the MEN1 gene. Direct sequence analysis of the exon 9 genomic DNA amplicon of proband II-4 (right) revealed a heterozygous G to A transition encoding the missense D418N mutation compared with an unrelated normal individual (left).