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Example smFRET kinetic traces. A–F, comparison of WT (A–C) and K180A (D), R74A (E), and R328A/R329A (F) EcDnaB FRET efficiencies as a function of time on the 30/30, 40/30, and 50/30 forks, respectively. The calculated FRET values (blue) are overlaid with the ideal state fits (red).
