Figure 3.

METH stimulates Thr-53 phosphorylation in vivo. A, male Sprague-Dawley rats were given subcutaneous injections of (A) saline, (–)-cocaine (15 mg/kg), or METH (15 mg/kg) and decapitated after 30 min, or (B) saline or METH (15 mg/kg) and decapitated at the indicated time points. Striatal membranes were harvested and equal amounts of protein analyzed for Thr-53 phosphorylation (upper panels) or total DAT (lower panels). Vertical white dividing lines in blots indicate the removal of intervening lanes from the same gel. Histograms show quantification of Thr-53 phosphorylation (% basal, mean ± S.E., *, p < 0.05; **, p < 0.01, n = 3–5); A, one-way ANOVA, with Tukey's post hoc test; B, Student's t test.