Figure 6.

[³H]CFT binding characteristics of Thr-53 mutants. WT, T53A, and T53D rDAT-LLCPK₁ cells were assessed for [³H]CFT saturation binding (A and B), or Zn²⁺ effects on [³H]CFT binding (C). A, [³H]CFT saturation binding plots. Each point represents the mean ± S.E. of three independent experiments. B, K_d values obtained from plots in A. *, p < 0.05, T53A versus T53D; **, p < 0.01, T53A versus WT; WT versus T53D not significant (ANOVA followed by Tukey's post hoc test). C, cells were incubated with vehicle or 20 μm ZnCl₂ followed by analysis of [³H]CFT binding. Values shown are mean ± S.E. of basal value for each form. ***, p < 0.001, Zn²⁺ versus vehicle; ##, p < 0.01, T53A and T53D DAT versus WT DAT at 20 μm Zn²⁺ (ANOVA followed by Tukey's post hoc test, n = 5).