. 2017 Sep 22;292(46):19066–19075. doi: 10.1074/jbc.M117.787002

Table 1.

Kinetic properties of DAT Thr-53 mutants

WT, T53A, and T53D DAT forms were analyzed by RDEV to determine Michaelis constant (K_m) and maximal velocity (V_max) for DA uptake and AMPH-induced DA efflux. Results are expressed as mean ± S.E. from n = 4 (T53A) or n = 5 (WT and T53D) independent experiments.

Cells	DA uptake		AMPH-induced DA efflux		V_max efflux/V_max uptake
Cells	V_max	K_m	V_max	K_m	V_max efflux/V_max uptake
	pmol/s/mg	μm	pmol/s/mg	μm
WT	28.2 ± 5.7	1.83 ± 0.38	15.2 ± 2.1	446 ± 116	0.72 ± 0.23
T53A	64.6 ± 10.9^a	3.05 ± 0.73	81.4 ± 6.7^a	743 ± 136^a	1.46 ± 0.17^a
T53D	43.8 ± 9.7	1.86 ± 0.34	25.2 ± 5.2^b	389 ± 60^b	0.49 ± 0.06^b

^a p < 0.05, T53A DAT vs. WT DAT.

^b p < 0.05, T53D DAT vs. T53A DAT (one-way ANOVA followed by Holm-Sidak post hoc test).