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. Author manuscript; available in PMC: 2017 Nov 28.
Published in final edited form as: J Cell Sci. 2012 Aug 16;125(Pt 21):5151–5158. doi: 10.1242/jcs.108498

Fig. 6. The PLC inhibitor, edelfosine, did not alter the ATP-mediated reduction in Ins(1,4,5)P3-evoked Ca2+ release in voltage-clamped single myocytes.

Fig. 6

(A) Photolysed caged Ins(1,4,5)P3 (▲) increased [Ca2+]c (i) as indicated by F/F0. ATP (1 mM by pressure ejection, applied 2 s before Ins(1,4,5)P3; ii) decreased Ins(1,4,5)P3-evoked [Ca2+]c increases. Edelfosine (10 μM, n=4) did not alter the inhibitory effect of ATP on Ins(1,4,5)P3-evoked Ca2+ release (VM –70 mV). (B) The Ins(1,4,5)P3-generating agonist, CCh (100–250 μM by pressure ejection; ii), increased [Ca2+]c (i) as indicated by F/F0. Edelfosine (10 μM) decreased CCh-evoked [Ca2+]c increases (i), which is consistent with the proposed mechanism of action of the drug. Edelfosine was perfused into the solution bathing the cells.