Table 1.

Levels of pH, un-ionized nicotine (%), moisture (%), total nicotine and un-ionized nicotine (mg/g) in rapé samples.

Product	pH1	% Un-ionized Nicotine	Moisture (%)1	Total Nicotine1 (wet, mg/g)	Un-ionized Nicotine2 (wet, mg/g)	Total Nicotine (dry, mg/g)	Total Nicotine (dry, %)
Rapé (manufactured, non-tobacco)
Rapé Cacitral	5.71	n.a.	7.65	<LOD	n.a.	n.a.	n.a.
Rapé Guarany Cristal	5.93	n.a.	5.77	<LOD	n.a.	n.a.	n.a.
Rapé (manufactured, tobacco-containing)
Rapé Guarany Especial	5.63	0.40	5.85	6.32	0.03	6.72	0.67
Rapé Guarany Especial (Canela)	5.73	0.52	6.66	6.72	0.03	7.20	0.72
Rapé Guarany Cravo	5.34	0.21	9.46	15.0	0.03	16.6	1.66
Rapé Moeda Especial	5.17	0.14	7.21	20.7	0.03	22.4	2.24
Rapé Caratinga Mentolado	6.42	2.46	5.56	21.6	0.53	22.9	2.29
Rapé Caratinga Imburana	6.23	1.59	6.15	24.2	0.39	25.8	2.58
Rapé Araçá Cravo e Canela	5.83	0.64	5.61	31.1	0.20	33.0	3.30
Rapé Moeda Girassol	5.78	0.57	6.21	32.7	0.19	34.8	3.48
Rapé Real (flavored)	5.91	0.77	5.80	44.7	0.34	47.5	4.75
Rapé Real (unflavored)3	5.98	0.91	6.51	47.6	0.43	50.9	5.09
Rapé (custom-made, tobacco-containing)
Rapé Kaxinawá	9.75	98.2	4.91	18.9	18.5	18.5	1.85
Rapé Nu-nu	10.2	99.3	3.68	15.5	15.4	15.4	1.54
Minimum Detected (tobacco-containing)	5.17	0.14	3.68	6.32	0.03	6.72	0.67
Maximum Detected (tobacco-containing)	10.2	99.3	9.46	47.6	18.5	50.9	5.09

¹Measurements were made in triplicate except for pH and % moisture (n=2).

²Total and un-ionzied nicotine was calculated using measured pH and total nicotine values. The % un-ionized is calculated using the Henderson-Hasselbalch equation.

³Based on nicotine values, FT/IR and GC/MS full scan analyses, Rapé Real (unflavored) was determined to be essentially pure Nicotiana rustica tobacco with no other discernable flavorings or plant material added.