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. Author manuscript; available in PMC: 2018 Jul 18.
Published in final edited form as: Biochemistry. 2017 Jul 3;56(28):3669–3681. doi: 10.1021/acs.biochem.7b00359

Figure 5. Current density in HEK293T/17 cells transfected with β2a, α2δ1 and WT or mutant α11.2 subunits fused to eCFP for identification of α11.2-expressing cells.

Figure 5

Electrophysiological recordings were ~48 hours post transfection. 300 ms depolarization pulses ranging from −40 mV to +80 mV with 10 mV intervals were given from a −70 mV holding potential. A) Summary I/V curves. Ba2+ currents (pA) are normalized by whole cell capacitance (pF) and are plotted as the function of voltage (pA/pF vs. mV). B) Panels depict sample traces ranging from −40 mV to +30 mV of WT (top) and Y1649 mutant (bottom) in corresponding color gradients (data for all mutants summarized in Table 1). C) Bar graph of mean current density at 20 mV (IBa2+, +/− S.E.M) in cells expressing either WT or the indicated mutant α11.2 CaV1.2 channels. Cells expressing α11.2 point mutants K1647A, Y1649A and I1654A exhibit significantly reduced current density when compared to WT, whereas the mutation F1648A and Q1655A did not show a significant reduction. (***= p<0.001, ANOVA, Tukey and Bonferroni post hoc tests; N=6–7).