. Author manuscript; available in PMC: 2018 Jul 18.

Published in final edited form as: Biochemistry. 2017 Jul 3;56(28):3669–3681. doi: 10.1021/acs.biochem.7b00359

Table 2.

Current density and curve fitting.

	pA/pF	V_1/2 (mV)	G_max	E_rev (mV)
WT	17.2±1.0	−1.1 ± 1.4	0.30 ± 0.02	72.8 ± 2.3
K1647A	4.4±1.1***	1.1 ± 1.4	0.09 ± 0.01	69.2 ± 1.3
F1648A	13.3±1.7	3.0 ± 1.5	0.26 ± 0.02	73.5 ± 1.7
Y1649A	3.9±0.4***	4.1 ± 2.7***	0.08 ± 0.01	66.5 ± 2.9
I 1654A	6.7±2.1***	11.2 ± 1.5***	0.15 ± 0.01	75.7 ± 1.7
Q1655A	12.8±1.1	−2.6 ± 1.6	0.25 ± 0.02	67.2 ± 2.7

Mean current density values at 20 mV (I_Ba2+, +/− S.E.M), half-activation voltage (V_1/2), maximum conductance (G_max), and equilibrium potential (E_rev) calculated from the Boltzmann IV equation (see Methods and Materials). Significant differences to WT means were found for K1647A, Y1649A, and I1654A (***p<0.001; ANOVA followed by Tukey post hoc test; N=6–7).