Figure 2. Immunohistochemical analysis of protein carbonylation in end-stage fNASH and nfNASH.
Paraffin embedded formalin fixed tissue sections were analyzed immunohistochemically using polyclonal antibodies directed against MDA (A–C), acrolein (D–F), 4-ONE (G–I) and 4-HNE (J–L). Figures are representative of hepatic tissue isolated from three normal, four nfNASH and four fNASH patients respectively.