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. Author manuscript; available in PMC: 2018 Jul 1.
Published in final edited form as: Anal Bioanal Chem. 2017 May 29;409(19):4615–4625. doi: 10.1007/s00216-017-0407-6

Fig. 1.

Fig. 1

Scheme of comparative sample processing workflows Proteins from HeLa cells were digested with trypsin and labeled with TMT reagent. Labeled peptides were then fractionated offline with a PFP column and acidic mobile phase (PFP), or a RP column and basic mobile phase (Hi-pH RP). Chromatograms are of TMT labeled peptides with fractional combinations overlaid; every 12th fraction was combined. Samples derived from RP fractionation were desalted prior to LC-MS/MS, whereas PFP fractions were not.