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Sialoglycan microarray binding specificity studies of human Siglec-9 (hSiglec-9-Fc, hSiglec-9), porcine torovirus hemagglutinin-esterase (PToV), and bovine coronavirus hemagglutinin-esterase (BCoV) (both PToV and BCoV were mutated to ablate their esterase activity) towards Neu5Ac9NAcα3Galβ4GlcβProNH2 (black columns), Neu5,9Ac2α3Galβ4GlcβProNH2 (gray columns), and Neu5Acα3Galβ4GlcβProNH2 (white columns) without esterase treatment (A) or treated with esterase active PToV (B).
