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Detection of incorporation of Neu5,9Ac2 or Neu5Ac9NAc into hypo-sialylated human lymphoma BJA-B K20 cells. Cells were fed (or not) for 3 days with free Sias (1 mM) and then stained with PToV in inactive (mutated, solid black line) or active (dashed black line) O-acetylesterase forms. The binding was analyzed by flow cytometry.
