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. Author manuscript; available in PMC: 2017 Nov 28.
Published in final edited form as: Biochem Biophys Res Commun. 2011 Sep 7;413(4):555–560. doi: 10.1016/j.bbrc.2011.08.137

Fig. 2.

Fig. 2

SSTR3 trafficking is regulated by Rab4. EGFP-SSTR3 expressing IMCD cells were transfected with mCherry Rab4 (A and B) or mCherry Rab4/S22N (C and D). After 48 h cells were imaged and consecutive images of cell regions outlined in A and C are presented in B and D, respectively. Arrowheads point to SSTR3 (green) separating from Rab4-containing (yellow) endosomes. Arrows point to SSTR3 only (green) elements separated from Rab4 only (red) elements. (E) SSTR3 separation from Rab4 or Rab4/S22N was measured by determining the co-localization coefficient between SSTR3 and Rab4 or Rab4/S22N as a function of time in 25 SSTR3/Rab4 or SSTR3/Rab4/S22N endosomes. Bars, 19 µm. (For interpretation of the references to color in this figure legend, the reader is referred to the web version of this article.)