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. Author manuscript; available in PMC: 2019 Feb 1.
Published in final edited form as: J Cell Physiol. 2017 Jun 22;233(2):1278–1290. doi: 10.1002/jcp.25996

Figure 4. CTCF is a structural component of the major histone locus body.

Figure 4

(A) ImmunoFISH was performed to 6p22 (red) and NPAT (green), a coregulatory protein involved in histone expression to demonstrate that NPAT can be used a surrogate for the major histone locus body (major HLB) within the nucleus. (B) Immunofluorescence before (CSK) and after (NIMF) nuclear matrix extraction shows that CTCF associates with the major HLB at the nuclear matrix. (C) ChIPseq for CTCF compared with looping interactions from the Hi-C demonstrates that CTCF is present at the anchor sites of looping interactions within the major HLB.