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Persistent display of 1 on Ext1−/− cells. (a) Detection of 1 on live Ext1−/− cell surfaces over time by flow cytometry. Cells were stained with avidin-AF488 and co-stained with PI to exclude nonviable cells. (b) Western blot analysis using an anti-biotin antibody of cell lysates after treatment with 1, neu. and ST6GAL1, and subsequent culturing in medium for the indicated time.
