FIG. 6.
Res increases SIRT1 and mitigates Th1 cytokine production in vitro. Spleen lymphocytes were stimulated with ConA (5 μg/mL) with or without Res (100 μM, 12 hours). (A) Western blot–assisted detection of SIRT1, p-Stat1, and Tbet. The intensity of the bands was normalized by dividing the target band intensity by that of β-actin. The values under the bands represent the relative ratio of normalized intensity compared with that of cells+ConA. Representations of 3 experiments are shown. (B) Quantitative RT-PCR–assisted detection of mRNA coding for SIRT1, IFNγ, and IL2. Expression levels were normalized to HPRT and normalized to the expression of cells+ConA (*P < 0.05 versus cells+ConA; n = 4; 1-way ANOVA). Data are presented as mean ± SD.