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OmpLA arginine mutants do not acquire PK resistance in wild-type E. coli at low temperature. MC4100 (A) or JMR352 (B) transformed with pMDG2 or a pMDG2 derivative encoding the indicated OmpLA mutant were shifted to 28° C and subjected to pulse-chase labeling after the addition of IPTG. The OM was permeabilized and half of each sample was treated with PK. Immunoprecipitations were then conducted using an anti-OmpLA antiserum.
