Fig. 8.

EspP mutants that contain a lipid facing arginine interact stably with the Bam complex. A and B. AD202 transformed with pDULE-Bpa and a derivative of pRI22 encoding wild-type EspP or EspP (G1123R), EspP (L1121R) or EspP (I1119R) with an amber mutation at position 1214 were subjected to pulse-chase labeling after the addition of IPTG. One aliquot of cells was UV-irradiated while an equal aliquot was left untreated. In part (A), PK was added to half of each sample. Immunoprecipitations were then conducted using the indicated antisera. The non-irradiated samples from part (A) are shown in Fig. S9.