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Survivin protein levels in the cytoplasmic and nuclear fractions measured by ELISA in mixed cultures of HCT116 WT (75%) and isogenicTP53 null (25%), and RKO WT (75%) and isogenic TP53 null (25%). P values were determined by comparing the protein content in the unirradiated control cytoplasmic (C) and nuclear (N) fractions with their corresponding groups from the two radiation protocols. Each experiment was repeated three times and error bars represent the SEM.
