Table 1. Information of primers for Real-time qPCR.
| Gene name |
NCBI Accession No. | Sequence (5’-3’) | Size (bp) | Annealing temperature (°C) | |
|---|---|---|---|---|---|
| Ifn-γ | NM_138880.2 | Forward Reverse |
CCCTCTCTGGCTGTTACTGC TTTCGTGTTACCGTCCTTTTG |
149 | 60 |
| Tnf-α | NM_012675.3 | Forward Reverse |
TCAGCCTCTTCTCATTCCTGC TTGGTGGTTTGCTACGACGTG |
203 | 60 |
| Tgf-β | NM_021578.2 | Forward Reverse |
CTGCTGACCCCCACTGATAC ACGTTTGGGACTGATCCCATT |
174 | 58 |
| Il-4 | NM_201270.1 | Forward Reverse |
GTACCGGGAACGGTATCCAC TGGTGTTCCTTGTTGCCGTA |
138 | 58 |
| Il-10 | NM_012854.2 | Forward Reverse |
TGCGACGCTGTCATCGATTT GTAGATGCCGGGTGGTTCAA |
186 | 58 |
| Il-17a | NM_001106897.1 | Forward Reverse |
TGGACTCTGAGCCGCAATGA CTCCACCCGGAAAGTGAAGG |
189 | 58 |
| Il-21 | NM_001108943.2 | Forward Reverse |
GCACGAAGCTTTTGCCTGTT CACAGGAAGGGCATTTAGCC |
161 | 58 |
| Il-22 | NM_001191988.1 | Forward Reverse |
CAACCGCACCTTTATGCTGG ATCCTTGGCTTTGACTCCTCG |
103 | 58 |
| Il-22ra2 | NM_001003404.1 | Forward Reverse |
CACTCCATGGTGGGAAACAAAA TCGAACATGGGCTGGTACAT |
291 | 58 |
| β-actin | NM_031144.3 | Forward Reverse |
GAGGGAAATCGTGCGTGAC GCATCGGAACCGCTCATT |
157 | 60 |