Fig 2. Effects of berry fruit extract (BFE) on adipogenesis induced in 3T3-L1 cells.

The effect of BFE on the viability (A), ROS production (B) and lipids accumulation (C–D) in 3T3-L1 adipocytes undergo the differentiation process. Microscopic images of adipocytes stained with Oil Red O (C) and the fat content measured after Oil Red O elution (D). Extract was added to the 3T3-L1 cell cultures at each stage of differentiation process at concentrations of 25, 50 and 100 μg/mL. Data are the mean values ± SD (n = 3). The results of a one-way ANOVA indicated significant effects of the extract on lipid concentration (F = 45.4, p < 0.0001) and ROS accumulation (F = 8.16, p < 0.0082) in differentiated fat cells. ^ap < 0.05, ^bp < 0.01, ^cp < 0.001 (Tukey’s post hoc test).