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. 2017 Nov 28;12(11):e0188221. doi: 10.1371/journal.pone.0188221

Fig 3. CD1d does not contribute to the induction of AAD in OVA-sensitized mice challenged with OVA.

Fig 3

Using 3 different approaches, BALB/c WT and CD1d- mice were inoculated i.p. with OVA/alum and then inhaled OVA. Panels A and B, i.p. injections were: PBS or 100 μg OVA + 4 mg alum on days 0 and 7, then airway challenges were: PBS or 100 μg OVA in 50 μl given daily intratracheally days 15–24. On day 27 airway responsiveness was measured invasively and BAL performed, Panels A and B respectively. Panels C and D, i.p. single injection was: PBS or 50 μg OVA + 4 mg alum on day 0, then airway challenges were: PBS or 50 μg OVA in 50 μl given intranasally days 8, 9 and 10. On day 11 airway responsiveness was measured invasively and BAL performed, Panels C and D respectively. Panels E and F, i.p. injections were: PBS or 20 μg OVA + 4.25 mg alum on days 0 and 14, then airway challenges were: 20 min exposures to aerosolized PBS or 1% OVA on days 28, 29 and 30. On day 31 airway responsiveness was measured invasively and BAL performed, Panels E and F respectively. Each set of panels depicts 2 pooled experiments; total number of mice/group was 6–9.