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. 2017 Nov 28;12(11):e0188221. doi: 10.1371/journal.pone.0188221

Fig 6. Anti-CD1d mAb effectively prevents responses to α-GalCer but does not prevent OVA-induced AHR.

Fig 6

Panel A, BALB/c WT mice were injected i.p. with 500 μg of anti-CD1d or isotype control mAb and treated i.t. with saline or 2 μg of α-GalCer. IL-4 production was measured by IVCCA during the subsequent 24 hrs. 1 experiment with 6 mice/group; a second experiment (not shown) gave compatible data. Panel B, BALB/c WT mice were injected i.p. with 500 μg of anti-CD1d or isotype control mAb, then challenged i.t. with 2 μg of α-GalCer and evaluated invasively 2 days later for AHR. 2 experiments pooled, 12 mice/group. Panel C, BALB/c WT mice were immunized i.p. with OVA/alum, then treated i.t. with saline or 12.5 μg of OVA on days 7, 8 and 9, then interrogated invasively for AHR on day 10. Additionally, the mice were injected i.p. with 500 μg of anti-CD1d or control mAb on days -1, 2, 5 and 9 (Before Sensitization groups) or on days 6 and 9 (After Sensitization groups). 2 experiments pooled 6–11 mice/group. * = p <0.05 as compared to saline-immunized mice. † = p < 0.05 as compared to anti-CD1d mAb-treated mice.