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. 2017 Nov 28;12(11):e0188886. doi: 10.1371/journal.pone.0188886

Fig 1. Identification of PUB12 in the PBL27 complex.

Fig 1

(A) The PBL27-3HA protein was detected in the membrane fraction. The total (T), soluble (S), and microsomal membrane (M) fractions purified from Arabidopsis seedlings expressing PBL27-3HA were used in immunoblots with α-HA. (B) PBL27 does not interact with PUB12 in the yeast two hybrid experiment. The growth of yeast colonies on the plate. (C) PUB12 interacts with the intracellular domain (IC) of CERK1, but not LYK5 in the yeast two hybrid experiment. The growth of yeast colonies on plates (-ULWH) lacking uracil (U), leucine (L), tryptophan (W), and histidine (H) with 2 mM 3-aminotriazole (3-AT) indicates a positive interaction.