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. 2017 Nov 28;12(11):e0188637. doi: 10.1371/journal.pone.0188637

Fig 1. Validation of miR205 targets.

Fig 1

(A) miR205 expression determined by a TaqManTM assay in SUM159_Control (Ctrl), and SUM159_miR205 (miR) (see Materials and Methods). Results are shown as mean ± SD of relative miR205 levels in three independent experiments in triplicate, considering arbitrarily the first sample of SUM159_Control (Ctrl) as 1 (**p<0.01). (B) EGFP-positive cells were isolated by FACS-Vantage SE from SUM159_miR205 infected with a bicistronic lentivirus containing Anti-miR205/EGFP to establish SUM159_miR205_Anti-miR205 cell line (see Materials and Methods). Result is shown as histogram from background (EGFP-) and positive populations (EGFP+) of sorted SUM159_miR205_Anti-miR205 cells. (C) Immunoblotting detection of VEGF-A, ErbB3, ZEB1, Fyn, and Lyn A/B from extracts of SUM159_Control (Ctrl), SUM159_miR205 (miR), SUM159_Control_Anti-miR205 (Ctrl_Anti-miR), and SUM159_miR205_Anti-miR205 (miR_Anti-miR) cells; GAPDH or β-actin were determined for loading controls. These are representative results from 3 independent experiments. The ratios referred to SUM159_Control (Ctrl) considered as 1.