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. 2017 Nov 28;12(11):e0188342. doi: 10.1371/journal.pone.0188342

Fig 3. Cytotoxic and degranulation potential, and perforin expression of NK/NKT-like cells during chikungunya against target cells (K562).

Fig 3

PBMCs (effector) were isolated from the whole blood of 12 chronic patients, 12 individuals recovered from chikungunya, and 12 controls. PBMCs were co-cultured with K562 (target) cells at effector/target ratio of 10:1 for 6 h to enumerate the percentage of CD107a on NK/NKT-like cells. Box plots show (A) % specific killing against target cells, (B) CD107a expression by NK cells, (C) CD107a expression by NKT-like cells, (D) perforin by NK cells, (E) perforin by NKT-like cells, (F) CD107a expression by NK cells in the chronic patients and controls before and after blocking with neutralization antibodies, and (G) CD107a expression by NKT-like cells in the chronic patients and controls before and after blocking with neutralization antibodies. Mann-Whitney U-test/Kolmogorov-Smirnov test was used for intergroup comparison. p value <0.05 is considered significant.