Table 3. Antigen capture ELISA in urine samples.

Sample Code	Serology	qPCR	Ag ELISA OD (plasma)*	Ag ELISA result (Plasma)	Ag ELISA OD (Urine)	Ag ELISA result (urine)
018R	Pos	Pos	0.4516	Pos	0.7164	Pos
074R	Pos	Pos	0.6831	Pos	1.1876	Pos
080R	Pos	Pos	0.5941	Pos	0.5934	Pos
094R	Neg	Neg	0.3638	Neg	0.2033	Neg
098R	Neg	Neg	0.4138	Neg	0.1198	Neg
V001	Neg	Neg	0.3300	Neg	0.4135	Neg
V002	Neg	Neg	0.2381	Neg	0.3506	Neg
V003	Neg	Neg	0.2993	Neg	0.3240	Neg
099R	Pos	Neg	0.6465	Pos	2.0799	Pos

Antigen capture ELISA was performed as describe in the text. The cut off values (mean plus two standard deviations) were 0.3816 for serum samples; 0.4395 for plasma samples; and 0.5195 for urine samples.

*Antigen capture ELISA for samples code V001—V003 were performed using serum samples and were obtained from volunteer individuals. Ag ELISA = antigen capture ELISA, OD = optical density, Pos = positive, Neg = negative.