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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Nat Protoc. 2017 Jul 27;12(8):1702–1721. doi: 10.1038/nprot.2017.058

Figure 4.

Figure 4

Semisynthesis of N-glycan oxazolines (Steps 1–44). (i) 15 mM NaIO4, PB, pH 7.0; (ii) N3(CH2)2ONH3+Cl− (21a), PB, pH 7.0; (iii) N3(CH2)3NH2, NaCNBH3, PB pH 6.0, MeOH; (iv) Endo-M, PB, pH 6.2–6.5; (v) DMC, Et3N; (vi) neuraminidase, PB, pH 5.0; (vii) β-1,4-galactosidase, PB, pH 6.5; (viii) β-N-acetylglucosaminidase, PB, pH 6.5.