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. 2017 Nov 28;6:e28951. doi: 10.7554/eLife.28951

Figure 1. Stages depicting the progression of heart looping in the mouse.

Figure 1.

(A) Schematic representation of shape changes during the formation and looping of the heart tube (orange) in the E8.5 mouse embryo. Until E8.5f, the mouse embryo appears bilaterally symmetrical, and the heart tube is straight. The staging scale goes from E8.5c to E8.5j (previous E8.5a-b stages are not shown). This scale, focused on the heart, is finer than Theiler stages, and not fully synchronous with the addition of somites. Somite numbers (nb) were counted in a collection of 40 embryos imaged by HREM between E8.5e and E8.5j and of 48 embryos between E8.5c and E8.5d observed under the microscope. (B). 3D reconstructions of heart shapes from HREM images at each stage of heart looping. All the reconstructions are aligned with the notochord vertical (green), the arterial and venous poles up and down, respectively. L, left; LA, left atrium; LV, left ventricle; OFT, outflow tract; R, right; RA, right atrium; RV, right ventricle. Scale bar: 100 μm.

Figure 1—source data 1. 3D reconstructions of heart stages during the looping process, in a 3D pdf format.
3D visualisation of the heart, as reconstructed from HREM images, at the six stages shown in Figure 1. This file should be opened with Adobe Acrobat Reader. Click on the image to activate the manual rotation of the reconstruction. Each image may be rotated at will with the mouse (hold left click). Zoom in and out with the mouse wheel. Shortcuts at the top align all images in a ventral or dorsal view, with the notochord vertical.
elife-28951-fig1-data1.pdf (20.9MB, pdf)
DOI: 10.7554/eLife.28951.004