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. Author manuscript; available in PMC: 2017 Nov 28.
Published in final edited form as: Biochem J. 2014 Sep 1;462(2):315–327. doi: 10.1042/BJ20140174

Figure 5. HEXIM regulates KDM5B expression.

Figure 5

(A) LNCaP cells stably transfected with control or HEXIM1miR were treated with vehicle, 10 nMR1881 or 10 μMbicalutamide (Bic) for 90 min. Results show ChIP analyses of lysates immunoprecipitated with antibodies against HEXIM1 or control non-specific rabbit immunoglobin and PCR amplification of the enhancer regions of CDK1 or CDC20. *P <0.05 relative to vehicle treated cells. (B) ChIP analyses of lysates from LNCaP cells immunoprecipitated with antibodies against HEXIM1 or control non-specific rabbit immunoglobin and PCR amplification of the −73339/−72922 region of KDM5B. (C) RNA was harvested from LNCaP cells stably transfected with control or HEXIM1miR and subjected to RT–PCR to assess KDM5B mRNA levels using GAPDH as control. bP <0.05 relative to control transfected cells with the same treatment. (D) Western blot analyses of endogenous KDM5B relative to GAPDH loading control in LNCaP cells stably transfected with control or HEXIM1miR. Figures are representative of at least three independent experiments. C, control.