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. Author manuscript; available in PMC: 2017 Nov 28.
Published in final edited form as: Biochem J. 2014 Sep 1;462(2):315–327. doi: 10.1042/BJ20140174

Figure 6. HEXIM1 regulates KDM5B recruitment.

Figure 6

LNCaP cells stably transfected with control or HEXIM1miR were treated with 10 μM bicalutamide for 90 min. (A) Western blot analyses of endogenous KDM5B or FLAG–KDM5B relative to the GAPDH loading control. ChIP analyses of lysates immunoprecipitated with antibodies against (B) H3K4me2 or (C) KDM5B or control non-specific rabbit immunoglobin and PCR amplification of the enhancer regions of CDK1 or GAPDH. The results are means±S.E.M. of three independent experiments. aP <0.05 relative to non-FLAG–KDM5B transfected cells and bP <0.05 relative to control miRNA transfected cells. (D) Lysates from LNCaP cells were immunoprecipitated (IP) using antibodies against HEXIM1 or KDM5B and analysed for co-immunoprecipitation of HEXIM1 or KDM5B by Western blotting. Normal rabbit IgG was used as a specificity control. Input lanes represent 25% of the total protein. Figures are representative of at least three independent experiments.