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. Author manuscript; available in PMC: 2017 Nov 28.
Published in final edited form as: Biochem J. 2014 Sep 1;462(2):315–327. doi: 10.1042/BJ20140174

Figure 7. Altered HEXIM1 expression results in altered cellular response to an anti-androgen.

Figure 7

(A) LNCaP cells stably transfected with control or HEXIM1miR were treated with vehicle, 1 nM R1881 and/or 1 μM bicalutamide for 4 h. RNA was harvested and subjected to RT–PCR to assess CDK1, CDC20, UBE2C and PSA mRNA levels using GAPDH as a control. (B) LNCaP cells were stably transfected with control or HEXIM1miR and processed for Western blot analyses of UBE2C and PSA levels. Figures are representative of at least three independent experiments. aP <0.05 relative to R1881 alone and bP <0.05 relative to control transfected cells with the same treatment.