FIG. 4.

Effect of transient high shear exposure on platelet P-selectin. Whole blood was perfused through the different constricted microfluidic channels, the effluent was collected and labeled with an Alexa-488 conjugated anti CD62P antibody or with an isotype-matched control mAb, and the expression level of P-selectin on the surface of platelets was analyzed by flow cytometry. To test the platelet reactivity following perfusion, some of the collected effluent from each device was incubated with TRAP. Levels. No significant difference in P-selectin expression was observed between the unsheared samples and the low shear (straight channel) or high shear samples. However, the level of P-selectin increased significantly after adding TRAP to the various matched samples. The data are presented as median ± SEM and were derived from five to nine experiments collected in duplicate each time. Statistical significance was assessed by comparing each group to each other using a one-way ANOVA with a Tukey's multiple comparison test: ***P < 0.0001.