FIG. 6.

Effect of high shear on platelet granule release. Whole blood was perfused through the constricted microfluidic devices, the effluents collected, and centrifuged to collect the plasma. The level of sP-selectin (a), sCXCL7 (b), and sPF4 (c) in the plasma samples was measured by ELISA. No significant difference in sP-selectin, sCXCL7, or sPF4 levels was observed between the unsheared samples and the low shear (straight channel) or high shear samples. However, treatment of the matched samples with TRAP showed a significant increase in the levels of SP-selectin, sCXCL7, and sPF4. The data are shown as the median value ± SEM and are representative of 5–6 independent experiments with different donors. The statistical significance was assessed by comparing each group using one-way ANOVA with a Tukey's multiple comparison test: *P < 0.0001.