Table 1.
Strains and plasmids used in this study.
| Strain | Parent | Genotype | Reference | |
|---|---|---|---|---|
| BY4742 | S288C | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | Brachmann et al., Yeast, 1998 | |
| DHY214 | BY4742 | MATα his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 SAL1+ CAT5(91M) MIP1(661T) MKT1(30G) RME1(INS-308A) TAO3(1493Q) HAP1+ | Chu and Horecka, Stanford Genome Technology Center | |
| S1 | JHY651* | DHY214 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 | ibid. |
| S2 | YJB011* | JHY651 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 | This study |
| S3 | YJB012* | JHY651 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye3Δ1 | This study |
| S4 | YJB013* | YJB011 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 | This study |
| S5 | YJB023* | YJB013 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 gre2Δ1 | This study |
| YJB025* | YJB013 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 ari1::LEU2 | This study | |
| S6 | YJB027* | YJB025 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 ari1Δ0 | This study |
| S7 | YJB018* | YJB013 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 adh6Δ1 | This study |
| YJB024* | YJB013 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 adh7::LEU2 | This study | |
| S8 | YJB052* | YJB024 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 adh7Δ0 | This study |
| YJB030* | YJB027 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 ari1Δ0 adh7::LEU2 | This study | |
| YJB033* | YJB030 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 ari1Δ0 adh7Δ0 | This study | |
| S9 | YJB051* | YJB033 | MATα prb1Δ pep4Δ his3Δ1 leu2Δ0 ura3Δ0 lys2Δ0 oye2Δ1 oye3Δ1 ari1Δ0 adh7Δ0 adh6Δ1 | This study |
| Plasmid | Description | Reference |
|---|---|---|
| pJB031 | 2μ; URA3; AmpR | This study |
| pJB033 | 2μ; URA3; AmpR; ADH2p-GOR-PRM9t | This study |
| pJB034 | 2μ; URA3; AmpR; ADH2p-GOR-PRM9t; PCK1p-ISY-CPS1t | This study |
| pJB046 | pCRCT-Δoye2 | This study |
| pJB047 | pCRCT-Δoye3 | This study |
| pJB057 | pCRCT-Δgre2 | This study |
| pJB053 | pCRCT-Δadh6 | This study |
| pJB097 | 2μ; kanMX; AmpR; iCas9; SNR52p-LEU2crRNA-sgRNA-SUP4t | This study |
| pJB042 | pET-28a-6×HIS-GOR | This study |
| pJB043 | pET-28a-6×HIS-ISY | This study |
| pJB044 | pET-28a-6×HIS-OYE2 | This study |
| pJB045 | pET-28a-6×HIS-OYE3 | This study |
| pJB072 | pET-28a-6×HIS-ADH6 | This study |
| pJB071 | pET-28a-6×HIS-ADH7 | This study |
| pJB095 | 2μ; URA3; AmpR; ADH2p-6xHIS-ARI1-PRM9t | This study |
Notes:
*
strain is also: SAL1+ CAT5(91M) MIP1(661T) MKT1(30G) RME1(INS-308A) TAO3(1493Q) HAP1+