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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Gastroenterology. 2017 Aug 30;153(6):1568–1580.e10. doi: 10.1053/j.gastro.2017.08.039

Figure 5. Knockdown of hepatic KCTD17 prevents diet-induced hepatic steatosis.

Figure 5

(A) qPCR and (B) western blots from primary hepatocytes transduced with Ad-shControl or Ad-shKctd17. (C) Knockdown of Kctd17 reduces endogenous PHLPP2 ubiquitination in HA/Ub-transfected primary hepatocytes. (D) Experimental outline, (E) western blots from liver lysate, (F) liver weight and (G) triglycerides, (H) liver H&E and Oil-Red-O staining, and (I) hepatic gene expression in C57BL/6 WT mice fed HFD for 14 weeks, then transduced with Ad-shControl or Ad-shKctd17 (n=6–7/group) prior to sacrifice at a total of 16 weeks HFD feeding. *P < .05, **P < .01, ***P < .001 as compared to the indicated control by two-way ANOVA. All data are shown as the means ± s.e.m.