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. Author manuscript; available in PMC: 2018 Nov 27.
Published in final edited form as: Annu Rev Genet. 2017 Aug 30;51:63–82. doi: 10.1146/annurev-genet-120116-024554

Figure 1.

Figure 1

Transgenic multicolor approach to visualize entire cell populations in live animals. (a) Schematic drawing of a Brainbow cassette. Each copy of a Brainbow cassette can result in one of three distinct colors after limited Cre-mediated recombination. In principle, the color choice is stochastically determined. (b) A high-copy number of Brainbow cassette can provide more color choices. For instance, transgenic animals with five Brainbow cassettes can theoretically generate twenty-one different colors to barcode cells of interest. (c) Brightfield view of an adult skinbow transgenic zebrafish. A red dashed-box indicates areas where the z-stacked confocal image shown below was captured. An entire population of skin epithelial cells is multicolor-barcoded. Scale bar, 1 mm. (d) A high-magnification view of skin surface cells in a skinbow zebrafish.