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. 2017 Jul 7;17(4):313–326. doi: 10.3727/105221617X15034976037343

Figure 8.

Figure 8

CWEC proliferation is reduced after inhibition of YAP activity with verteporfin (VP) and YAP knockdown in vitro. (A) Effects of different concentrations of VP on cell proliferation. CWECs were treated with the indicated VP concentrations with or without forskolin (FSK). DMSO was used as control. (B) Real-time PCR analysis of Birc5 transcripts after VP treatment. *Significant decrease (p < 0.05) relative to the DMSO; #significant decrease (p < 0.05) relative to FSK. (C, D) Western blotting analysis of total YAP protein after YAP short hairpin (shRNA)-mediated knockdown. The YAP band intensity was normalized to β-actin. (E) Effect of YAP knockdown on CWEC proliferation. Each experiment was repeated three times (CWECs from three separate biliary trees from three separate rats isolated on 3 separate days). The error bars represent the standard error of mean. *Significant decrease (p < 0.05) relative to the no shRNA treatment group; #significant decrease (p < 0.05) relative to control shRNA.