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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Matrix Biol. 2017 Jun 7;64:69–80. doi: 10.1016/j.matbio.2017.06.001

Figure 4.

Figure 4

Blocking cell attachment to fibronectin inhibits cell spreading in fibrin ECM. A) RGD peptide blocks cell spreading in fibrin matrices but not in collagen matrices. Representative pictures of collagen and fibrin matrices cultured overnight with RGD and RGE (control) peptide, and fixed and labeled for F-actin. For collagen, no difference in the pattern and amount of cell spreading was found between the three conditions. For fibrin, cells cultured with RGD peptide were not able to spread and remained rounded. Images are maximal intensity projections. Scale bar is 50 µm. B) Statistical analysis of cell spreading. Cell area was reduced in the presence of RGD. Data are mean ± standard deviation of 3 separate experiments. (*P<0.05, ANOVA on Ranks). C) Representative pictures of cells in fibrin matrices cultured with PDGF media containing antibodies that block fibronectin attachment (HFN 7.1) or α5β1 integrin attachment (P1D6). After overnight incubation, samples were fixed and stained for F-actin. Cells cultured with blocking antibodies were not able to spread and remained rounded. Images show maximum intensity projections. Scale bar is 50 µm.