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. Author manuscript; available in PMC: 2018 Aug 1.
Published in final edited form as: Curr Opin Microbiol. 2017 Jun 15;38:156–164. doi: 10.1016/j.mib.2017.05.006

Figure 4.

Figure 4

Applying CRISPR-based tools for genome editing and gene regulation. (A) Cas9 genome editing occurs through repair of the Cas9-mediated double-stranded break or through killing caused by an irreparable double-stranded break. (B) The CREATE method of high-throughput genome editing. (C) dCas9-mediated gene repression is achieved by introducing two point mutations to catalytically deactivate Cas9 (dCas9) and using it to block transcriptional initiation or elongation. (D) High-throughput genetic screen with dCas9-mediated gene repression.