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. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Gastroenterology. 2017 Aug 25;153(6):1674–1686. doi: 10.1053/j.gastro.2017.08.036

Figure 2. Ethanol and CSE in combination induce marked activation of the PERK pathway in acinar cells.

Figure 2

(A) AR42J cells were left untreated (C) or treated for the indicated times with EtOH, (50 mM) and/or CSE (40 μg/ml, left panel; 20 or 40 μg/ml, right panel). Expression levels of the UPR markers phospho-PERK, phospho-eIF2α, and GRP78 were assessed by Western blotting. Blots are representative of 3 independent experiments. (B) Densitometry analysis of immunoblots from (A), 48 h treatment. Levels (as a fraction of control values) were normalized to those of ERK1/2. Graph shows mean±SEM, n=3; * P<.05 vs. control. (C) Mouse pancreatic acini were treated for 24 h with 50 mM EtOH and/or 40 μg/ml CSE. Immunoblots show protein levels of phospho-PERK, phospho-eIF2α, GRP78, and ERK1/2.