Skip to main content
. Author manuscript; available in PMC: 2018 Dec 1.
Published in final edited form as: Peptides. 2017 Jun 3;98:78–85. doi: 10.1016/j.peptides.2017.05.011

Fig. 4.

Fig. 4

Vector construction for generating transgenic mosquitoes with AaeALP1 gene knockdown. (A) Construction of the mosquito transformation vector. The hairpin dsRNA construct of Aedes ALP1 was made with these components in order –heat-inducible Aedes heat shock 70A promoter, AaeALP1 inverted repeat (IR) left arm, APN intron (Lee et al. 2015), AaeALP1 IR right arm and SV40 terminator. This construct was cloned into pBAC[3xP3-EGFP afm] transformation vector containing 3xP3 (eye-specific promoter), TATA box, and enhanced GFP.(B) AaeALP1 gene transcript level detection by real time PCR. Orlando:a wild-type Ae. Aegypti mosquito strain used for embryo injections. F18: the stable transgenic mosquito strain with ALP1 invert repeat insert, that leads to ALP1 knockdown.