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. Author manuscript; available in PMC: 2019 Mar 1.
Published in final edited form as: J Cell Physiol. 2017 Aug 28;233(3):2343–2359. doi: 10.1002/jcp.26106

Fig. 7. Release analysis of the mutants on hydrophobic surface 1.

Fig. 7

(A) Determining the expression levels of the mutants (L29A, L32A and L36A) by Western blot as described for Fig. 5A. (B) Quantification of the bands for Agno on Western blots on panel A as described for Fig. 5B. (C) In parallel to WCE preparations for panel A, the supernatants collected from the transfected and untransfected cells were analyzed for Agno release by Western blot as described for Fig. 5C. (E) Quantification of the bands on Western blots for panel C as described for Fig. 5D.