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. Author manuscript; available in PMC: 2019 Jan 1.
Published in final edited form as: J Cell Biochem. 2017 Aug 3;119(1):824–836. doi: 10.1002/jcb.26246

Figure 3. Tyrosine phosphorylation regulates YAP localization in CCA.

Figure 3

A, cell lysates from the HuCCT-1 cell line were subjected to immunoblot for phosphorylated YAP (S127 and Y357) and YAP. Actin was used as a loading control. B, cell lysates from HuCCT-1 cell line were fractionated and subjected to immunoblot for phosphorylated YAP (S127 and Y357) and YAP. Lamin B1 and GAPDH were used as loading controls. C, immunofluorescence images from the SB-1a CCA cell line ± PDGFR inhibitor crenolanib (10 μM) with quantification of subcellular localization (right panel). Scale bar = 10 microns.