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Relative fold expression of cylA in eight clinical E. faecalis isolates. The expression levels of cylA were determined by qRT-PCR, with the clinical E. faecalis isolate 16C281 as the reference strain (expression = 1, biofilm negative). Biofilm phenotype: –, negative; +, weak; ++, medium; +++, strong. The cylA was overexpressed in the five weak biofilm strains (4.02 to 6.00-fold).
