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PCR primers used for determining the expression levels of esp and cylA of E. faecalis isolates by quantitative real-time polymerase chain reaction (qRT-PCR).
| Target | Primer | Primer sequence (5′–3′) | Amplicon size (bp) | Reference |
|---|---|---|---|---|
| recA | recA-F | CGACTAATGTCTCAAGCACTAC | 106 | This study |
| recA-R | CGAACATCACGCCAACTT | |||
| esp | Resp-F | GCATCAGTATTAGTTGGT | 196 | This study |
| Resp-R | TTCCTTGTAACACATCAC | |||
| cylA | RcylA-F | GGAGGATATGGTGACAAT | 163 | This study |
| RcylA-R | TTACTTCTGGAGTTGCTAA |
