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. 2017 Nov 24;8:807. doi: 10.3389/fphar.2017.00807

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Copyright © 2017 Mühlhaus, Dinter, Jyrch, Teumer, Jacobi, Homuth, Kühnen, Wiegand, Grüters, Völzke, Raile, Kleinau, Krude and Biebermann.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Gs signaling properties of TAAR1 wild-type and TAAR1 variants R23C, S49L, and I171L after stimulation with T1AM. For determination of Gs signaling properties, HEK293 cells, transiently transfected with TAAR1 wild-type (TAAR1-WT) and TAAR1 variants, were stimulated with T1AM (10^-9M – 10^-5M) and cAMP accumulation was measured with the AlphaScreen technology. Cells transfected with empty vector (mock) served as a negative control. Data are represented as mean ± SEM of fold over basal of the respective receptor variant and are based on 5–7 independent experiments performed in triplicates. Significance was calculated using one-way ANOVA; ^∗p ≤ 0.05, ^∗∗∗p ≤ 0.001. To mimic the (A) homozygous state and the (B) heterozygous state, cells were transfected with TAAR1-WT, TAAR1-R23C, TAAR1-S49L, or TAAR1-I171L.