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. 2017 Nov 24;8:1627. doi: 10.3389/fimmu.2017.01627

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Copyright © 2017 Jaigirdar, Benson, Elmesmari, Kurowska-Stolarska, McInnes, Garside and MacLeod.

This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) or licensor are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.

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Lipopolysaccharide (LPS) injection into the ear causes extensive cellular infiltrate. 24 h after the injection of PBS or LPS into ear pinnae, the response was examined by microscopy (A), digital calipers (B), or flow cytometry (C–F). Representative images of H&E stained sections (A) or homogenized ears (C,D) are shown. In panel (A), the scale bar shows 200 μm. In panel (B), an average of three individual measurements was taken for each ear pinnae. In panels (C,E), cells are gated on live cells, in panel (D) on live CD45+ CD11c+ cells and in panel (F) on live CD45+ CD11c+ MHCII+ cells. Data are representative of two independent experiments with three to five animals per group. Error bars show SEM.